E-Poster Presentation Australian Society for Microbiology Annual Scientific Meeting 2021

Microplates and machine-learning – determining antimicrobial efficacy and resistance through UV-Visible spectroscopy   (#360)

Rebecca Orrell-Trigg 1 , Sheeana Gangadoo 1 , Daniel Cozzolino 2 , Vi Khanh Truong 1 , James Chapman 1
  1. RMIT University, Melbourne, VICTORIA, Australia
  2. Centre for Nutrition and Food Sciences, The University of Queensland, Brisbane, QLD, Australia

Antimicrobial resistance is a growing problem for global health as more pathogens become resistant to existing antibiotics [1]. Nanomaterials such as graphene oxide [2] are promising antimicrobial agents to which microbes have less natural resistance than standard antimicrobial drugs. Current laboratory antimicrobial testing techniques can be insufficient for testing large quantities of potential materials, leading to antimicrobial studies taking far longer than desired. UV-Visible spectroscopy, coupled with machine learning, has the potential to shorten this process dramatically and allow for rapid testing of a multitude of samples for antimicrobial efficacy and the development of antimicrobial resistance [3]. This system of an automated microplate UV-Visible spectrometer and machine-learning techniques has been tested with both organic and inorganic materials and a range of bacteria including model organisms of E. coli and Methicillin-resistant S. aureus and shows great promise in being a new rapid technique for testing the chemical interaction between bacteria and antibiotics. 

  1. 1. World Health Organisation., Global Action Plan on Antimicrobial Resistance. 2015: Geneva.
  2. 2. Rajapaksha, P., et al., Antibacterial Properties of Graphene Oxide–Copper Oxide Nanoparticle Nanocomposites. ACS Applied Bio Materials, 2019. 2(12): p. 5687-5696.
  3. 3. Chapman, J., et al., A high-throughput and machine learning resistance monitoring system to determine the point of resistance for Escherichia coli with tetracycline: Combining UV-visible spectrophotometry with principal component analysis. Biotechnology and Bioengineering, 2021. 118(4): p. 1511-1519.